Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Sep 1 2011
Last update date: Apr 6 2016
Access: Public
Taxon: Bacillus subtilis
Dataset link RNA processing in Bacillus subtilis: Identification of targets of the essential RNase Y

Experimental Protocol


To study the global function of RNase Y, a microarray analysis was performed with a B. subtilis strain allowing controlled depletion of RNase Y. Strain GP193 (Commichau et al., 2009, Mol. Cell. Proteomics 8: 1350-1360) expressing the rny gene under the control of a xylose-inducible promoter was cultivated in CSE minimal medium in the presence or absence of the inducer xylose. The transcriptomes of the two cultures (i.e. expressing RNase Y and depleted for RNase Y) were compared at a timepoint at which the reduced RNase Y amounts already affected the mRNA turnover whereas the growth rates of the two cultures were still almost identical.

Repositories


GEO

GSE30430

ArrayExpress

E-GEOD-30430

BioProject

PRJNA143531

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Contact


Ulrike Mäder
Ulrike Mäder