|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Sep 1 2011|
|Last update date:||Apr 6 2016|
|Dataset link||RNA processing in Bacillus subtilis: Identification of targets of the essential RNase Y|
To study the global function of RNase Y, a microarray analysis was performed with a B. subtilis strain allowing controlled depletion of RNase Y. Strain GP193 (Commichau et al., 2009, Mol. Cell. Proteomics 8: 1350-1360) expressing the rny gene under the control of a xylose-inducible promoter was cultivated in CSE minimal medium in the presence or absence of the inducer xylose. The transcriptomes of the two cultures (i.e. expressing RNase Y and depleted for RNase Y) were compared at a timepoint at which the reduced RNase Y amounts already affected the mRNA turnover whereas the growth rates of the two cultures were still almost identical.