Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Oct 18 2007
Last update date: Dec 6 2012
Access: Public
Mutations: g|SUB|G|4131|A
Computational protocol: limmaGUI, MetaCore, ErmineJ, GenMAPP, Beacon Designer, GEO, BioCarta, KEGG
Dataset link Colonic gene expression upon Salmonella infection and dietary treatment

Experimental Protocol


In the present study, large-scale gene expression analysis was performed to reveal whether Salmonella induced changes of colonic mucosal gene expression in rats. Furthermore, we compared the colonic gene expression changes of infected rats fed a diet supplemented with Fructo oligosaccharides (FOS) or cellulose as control. Two groups of Wistar rats (n=12) were adapted for 14 days to a cellulose diet and one group (n=12) to a FOS diet. One cellulose-fed group and the FOS-fed group were infected with Salmonella. RNA was isolated from colonic mucosal scrapings. mRNA samples of 12 rats per group were pooled. Each group-sample was hybridised in duplicate on Agilent rat whole genome microarrays containing 44290 60-mer spots.

Repositories


GEO

GSE7472

ArrayExpress

E-GEOD-7472

BioProject

PRJNA100275

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Contact


Wendy Rodenburg

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