Computational protocol: Monoclonal antibody humanness score and its applications

Similar protocols

Protocol publication

[…] An input VH, VK, or VL variable region protein sequence is first assigned Kabat numbering and CDR residues are identified. The full-length sequence or the framework only sequence (with CDR residues removed) is compared to every sequence in the respective antibody database using the blastp protein-protein BLAST algorithm []. The sequence identity between each pairwise comparison is isolated, and after every sequence in the database has been analyzed, the sequences are sorted from high to low based on the sequence identity to the input sequence. The percent identity of the Top 20 matched sequences is averaged to obtain the T20 score. The T20 score analyzer was coded entirely using Python. […]

Pipeline specifications

Software tools BLASTP, DELTA-BLAST
Application Amino acid sequence alignment
Organisms Homo sapiens
Diseases Autoimmune Diseases, Neoplasms, Nervous System Diseases