Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 20
Release date: Dec 25 2009
Last update date: Mar 21 2012
Access: Public
Diseases: Mastitis
Dataset link The effect of negative energy balance on bovine blood PMN gene expression

Experimental Protocol


Briefly, 10 multiparous Holstein cows in PEB and past peak lactation were used for this study. The NEB cows were feed-restricted to 60% of calculated NEL requirements for 7 d, whereas PEB cows were fed the same diet for ad libitum intake. After 5 d of feed restriction, one rear mammary quarter of each cow was inoculated with 5,000 cfu of Streptococcus uberis (strain O140J). Blood PMN were isolated at 24 h post-inoculation from all cows. A 13,257-oligonucleotide (70-mers) array was used for transcript profiling. Cy3- and Cy5 labelled cDNA from PMN and a reference standard were used for hybridizations. All samples were hybridized to duplicate slides with reverse labeling (20 microarrays total).

Repositories


GEO

GSE15364

ArrayExpress

E-GEOD-15364

BioProject

PRJNA117095

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Contact


Kasey Moyes
Kasey M Moyes