Dataset features


Dataset type: Other
Number of samples: 19
Release date: Nov 30 2007
Last update date: Mar 17 2012
Access: Public
Genes: ISW2, INO80, INO80
Taxon: Saccharomyces cerevisiae
Dataset link Yeast replication in the presence of MMS: WT and isw2 nhp10 strains

Experimental Protocol

Genomic DNA isolated from S.cerevisiae samples taken at 30, 45, 60, 90, and 120 minutes (wild type) or 45, 60, 90, 120, and 150 minutes (isw2 nhp10) after release from G1 arrest (in media containing 0.015% MMS) was digested by restriction enzyme and fractionated into 2 pools: DNA that was replicated in the latest S-phase, and DNA that had not yet been replicated. DNA from each pool was oppositely labeled and co-hybridized to a microarray. 19 total samples: 10 for WT collection 9 for isw2 nhp10 collection All samples were from the same 2 experiments (1 WT collection, 1 isw2 nhp10 collection) Each sample has one dye swap pair (with the exception of the 45 min sample from the isw2 nhp10 collection). Dye swapped samples are named identically until the final character (either a or b) Percent replication values were determined for each corresponding spot on the array as described within.








Jack Vincent