Computational protocol: Regional Amyloid Deposition in Amnestic Mild Cognitive Impairment and Alzheimer's Disease Evaluated by [18F]AV-45 Positron Emission Tomography in Chinese Population

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Protocol publication

[…] Both PET and MRI images from each subject were co-registered first, and then MRI images were spatially normalized into the MNI T1 template as in Statistical Parametric Mapping 5 (SPM5) (Wellcome Department of Cognitive Neurology, University College London, London, UK). The transformation parameters of MR normalization were then applied to corresponding PET images. All the image data were processed in the PMOD software (version 3.2; PMOD Technologies Ltd., Zurich, Switzerland). For all subjects, a binary gray matter mask was first created by taking the non-zero pixels from an averaged gray matter probability map generated from the segmentation of the spatially normalized MR images from normal subjects. The final VOI template for VOI analysis was then obtained by taking the intersection of both the binary gray matter mask and the automated anatomic labeling (AAL) atlas (VOI template) for all subjects , ; this step was performed to minimize the inclusion of both cerebrospinal fluid and white matter (and thus non-specific white matter [18F]AV-45 retention) in the statistical measures of all VOIs . The original AAL atlas which contains 116 regions was combined into the following 8 labeled VOIs for further analysis: the precuneus, frontal, parietal, occipital, temporal, posterior cingulate and anterior cingulate areas, and the whole cerebellum. The standard uptake value ratio (SUVR) was calculated by determining the ratios of the integrated activities between the target VOI (cortical regions) and whole cerebellum reference VOI.Voxel-based statistical analysis was conducted with SPM5 . The spatial normalization of individual [18F]AV-45 images to MNI MRI T1-template space was performed in the same way as that for the VOI analysis described above. All voxels in the normalized [18F]AV-45 images were divided by the mean [18F]AV-45 uptake of the whole cerebellum VOI in each subject to form SUVR images. Moreover, all PET images in SPM analysis were smoothed by 8 mm FWHM Gaussian filtering. Voxel-wise [18F]AV-45 uptake differences among the CN, aMCI, and AD subjects were assessed in SPM5. Statistical maps displaying group differences were shown at a significance value of p<0.01 and extent voxels of 100. Due to a relatively small sample size of our study, and to increase the sensitivity in the SPM analysis, the lower threshold of two-tailed p<0.01 under uncorrected statistics was applied here . […]

Pipeline specifications

Software tools SPM, AAL
Application Magnetic resonance imaging
Diseases Alzheimer Disease, Amyloidosis