Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Oct 1 2013
Last update date: Aug 23 2018
Access: Public
Diseases: Adenocarcinoma, Adenoma, Carcinoma, Intestinal Neoplasms, Neoplasms, Tumor Lysis Syndrome
Dataset link Expression data from subpopulations of Apc1638N/+ intestinal adeno tumors versus Apc1638N/+ / KRAS v12G intestinal adenocarcinomas tumors

Experimental Protocol


To identify molecular differences between stem-like and more differentiated (bulk) tumour cells from Apc1638N/+ and Apc1638N/+/KRASV12G intestinal adenomas and adenocarcinomas, we isolated total RNA from 104 Lin-CD24hiCD29+, Lin-CD24medCD29+/Lin-CD24loCD29+ and Lin- (bulk) tumour cells from 5 individual mice of each genotype (Apc1638N/+ and Apc1638N/+/KRASV12G). Total RNA samples were then employed to hybridize oligonucleotide microarrays (Affymetrix Mouse Genome 430A 2.0 Array) according to conventional protocols.

Repositories


GEO

GSE47772

ArrayExpress

E-GEOD-47772

BioProject

PRJNA207775

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Contact


Andreas Kremer

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