Computational protocol: Age-Dependent Effect of Pediatric Cardiac Progenitor Cells After Juvenile Heart Failure

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Protocol publication

[…] RNA was extracted and whole gene and microRNA (miRNA) array was performed on three hCPC cell lines from three different patients in each group: neonate, infant, and child. Affymetrix gene arrays were performed by the Emory Integrated Genomics Core. Principal component (PC) analysis and partial least squares regression (PLSR) were performed using SIMCA-P software (UMetrics, UmeĆ„, Sweden, that solves the PLSR problem with the nonlinear partial least squares algorithm. Gene data were analyzed by Metacore and ingenuity pathway analysis. […]

Pipeline specifications

Software tools MetaCore, IPA
Application miRNA array analysis
Organisms Rattus norvegicus, Homo sapiens
Diseases Heart Defects, Congenital, Heart Diseases, Heart Failure