Adipocyte Differentiation of Immortalized Human Bone Marrow-derived Stromal Cells is Modulated by microRNA-155, microRNA-221 and microRNA-222

The telomerase immortalized human bone marrow-derived stromal cell line hMSC-Tert20 was differentiated towards adipocytes, and total RNA was harvested at various time points (0, 8, 24, 32, 48 and 72 hours, and 7, 14 and 21 days). All hybridizations, except for 0h, 8h and 32h, were performed twice. The expression of each miRNA at a given time point was calculated as a ratio relative to its level on day 0. No dye swaps.