Dataset features
Specifications
Application: | Gene expression microarray analysis, miRNA array analysis |
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Number of samples: | 15 |
Release date: | Aug 23 2011 |
Last update date: | Apr 17 2012 |
Access: | Public |
Genes: | MIR155, MIR221, MIR222 |
Dataset link | Adipocyte Differentiation of Immortalized Human Bone Marrow-derived Stromal Cells is Modulated by microRNA-155, microRNA-221 and microRNA-222 |
Experimental Protocol
The telomerase immortalized human bone marrow-derived stromal cell line hMSC-Tert20 was differentiated towards adipocytes, and total RNA was harvested at various time points (0, 8, 24, 32, 48 and 72 hours, and 7, 14 and 21 days).
All hybridizations, except for 0h, 8h and 32h, were performed twice. The expression of each miRNA at a given time point was calculated as a ratio relative to its level on day 0. No dye swaps.
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Heidi Namløs
Heidi Maria Namløs
Heidi Namløs