Dataset features


Application: Gene expression microarray analysis
Number of samples: 9
Release date: Dec 29 2017
Last update date: Jul 26 2018
Access: Public
Diseases: Immune System Diseases
Dataset link Expression data from human iNKT cells with or without interaction with human airway epithelium

Experimental Protocol

Human iNKT cells (an in vitro expanded clone [LH22]; Ho et al, 2004) were selected at three conditions for RNA extraction and hybridization on Affymetrix Gene ST 1.0 microarrays. For baseline, we used iNKT cells which had not been exposed to airway epithelium ('untouched' - iNKT_0h). Then, we studied the biological processes that occur in iNKT cells after interaction with human airway epithelium. For this, we pulsed normal human bronchial epithelial (NHBE) cells with αGC for 24 hours, then co-cultured these with iNKT cells directly (‘Direct’ - iNKT_Direct_4h) or separated by Transwell™ inserts (‘Transwell’ - iNKT_Transwell_4h). Three biological replicates were studied at each condition.