Computational protocol: Outbreaks of epidemic keratoconjunctivitis caused by human adenovirus type 8 in the Tibet Autonomous Region of China in 2016

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Protocol publication

[…] Editing and splicing of sequence data were performed using Sequencher software (Version 5.4.5, Gene Codes Corporation, USA). Basic Local Alignment Search Tool (BLASTn program; National Center for Biotechnology Information, Bethesda, MD, USA) was used to identify homologous nucleotide sequences in the GenBank database. Sequence alignment and phylogenetic trees were created using MEGA software (Version 5.0.3) []. Phylogenies were constructed using a maximum likelihood method with Tamura-Nei model; other parameters in the MEGA software were set to default. […]

Pipeline specifications

Software tools Sequencher, BLASTN, MEGA
Application Phylogenetics
Organisms Homo sapiens
Diseases Adenoviridae Infections, Keratoconjunctivitis