Computational protocol: Visualization of Radial Peripapillary Capillaries Using Optical Coherence Tomography Angiography: The Effect of Image Averaging

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Protocol publication

[…] The most superficial OCTA slab (50 μm deep from the inner limiting membrane [ILM]) () from AngioVue was used for image analysis in order to isolate only the most superficial layer of RPCs. For each of the eyes imaged, the OCTA with the highest contrast and the least motion artifact (i.e. fewest vertical and horizontal artifactual lines) was selected as the reference image; the other nine OCTAs were registered to this reference image using the Register Virtual Stack Slices plug-in on Image J; we employed a rigid extraction model with elastic bUnwarpJ splines registration []. For sets of images in which registration failed using the superficial OCTA slab due to low contrast, a deeper layer with larger blood vessels (vitreous to 150 μm below the ILM) was registered, and the transformations from this set of deep layer images was then applied to the superficial OCTAs using the Transform Virtual Stack Slices plug-in on ImageJ. […]

Pipeline specifications

Software tools Register Virtual Stack Slices, bUnwarpJ, ImageJ
Application Microscopic phenotype analysis
Organisms Homo sapiens
Diseases Eye Abnormalities, Glaucoma, Radial Neuropathy