Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 15
Release date: Nov 27 2013
Last update date: Sep 12 2014
Access: Public
Chemicals: Arabinose, Carbohydrates, Carbon
Dataset link A comparative systems analysis of polysaccharide-elicited responses in Neurospora crassa reveals carbon source-specific cellular adaptations

Experimental Protocol


Our data are based on carbon source transfer experiments. For this, N. crassa pregrown in sucrose for 16 hrs was washed in media without carbon and then transferred to either pectin, orange peel powder (OPP), xylan, Avicel (cellulose), sucrose, or media without carbon (NoC) for another 4 hrs. Biological triplicates (pectin, Avicel, sucrose, NoC) or duplicates (OPP) were used to identify differentially expressed genes in WT. For xylan only a single library was prepared, which was found to correlate well with previously published microarray data (Sun et al. 2012; doi: 10.1128/EC.05327-11)

Repositories


GEO

GSE42692

ArrayExpress

E-GEOD-42692

ENA

SRP017397

BioProject

PRJNA182734

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Contact


Johan Benz
Johan Philipp Benz