Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Aug 14 2012
Last update date: Aug 12 2017
Access: Public
Diseases: Encephalitis, California, Infection
Dataset link Comparative transcriptomic analysis of acute host responses during 2009 pandemic H1N1 influenza infection in mouse, macaque, and swine (macaque dataset)

Experimental Protocol


The goal of this experiment was to use global gene expression profiling to understand non-human primate lung cellular responses to pandemic H1N1 influenza A/California/04/2009 virus infection. Four- to fifteen-year-old cynomologous macaques were infected with CA04 virus (n = 4) under anesthesia through a combination of intratracheal (4 ml), intranasal (0.5 ml per nostril), conjunctival (0.5 ml per eyelid) and oral (1 ml) routes with a suspension containing 10^6 TCID50/ml (total infectious dose was 7x10^6 TCID50). Animals were euthanized on 1 and 6 days post-inoculation (n = 2 per time point), and total RNA was extracted from lung samples and analyzed by microarray. Pooled RNA from lungs of uninfected animals served as the reference.

Repositories


GEO

GSE40088

ArrayExpress

E-GEOD-40088

BioProject

PRJNA172764

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Contact


Michael Katze
Richard Roy Green