|Application:||Gene expression microarray analysis|
|Number of samples:||24|
|Release date:||Aug 25 2011|
|Last update date:||Aug 28 2018|
|Dataset link||Gene expression response to bacterial phagocytosis by S2 cells (control and eater RNAi knock down)|
Drosophila S2 cells, a hemocyte-derived cell line with phagocytic properties, were exposed to a mixture of Gram-positive and Gram-negative bacteria at conditions of 50% of cell binding. At different time points of synchronized phagocytosis (30, 90 and 180 minutes) total RNA was extracted and subjected to microarray analysis. We compared S2 cells in which Eater expression was decreased by RNA interference with control S2 cells exposed to irrelevant double-stranded (pBR322). Three independent experiments were performed corresponding to three biological replicates. Decreased phagocytosis due to RNAi eater knock down was controlled in all cases.