Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 6
Release date: May 14 2014
Last update date: Oct 10 2018
Access: Public
Computational protocol: Bowtie, HOMER, GEO
Dataset link Specific mitotic chromatin association of the major notch effector RBPJ and its implication for transcriptional memory

Experimental Protocol


To compare the genomic RBPJ localization in mitotic and interphase cells, mouse F9 cells were harvested and labeled as cycling cells (containing 95% interphase and 5% mitosis cells); nocodazole treated F9 cells were harvested and labeled as mitotic cells. Cell samples were proceeded to ChIP-seq experiments, and each of the experiment contains a set of ChIP DNA product: input as the background control and IP as the RBPJ binding product. Background noise was substracted and the obtained signal was used for the comparison of interphase and mitosis by statistical analysis. Please note that processed data (*bed) was generated from *rep1 sample (i.e. no processed-data for rep2 sample).

Repositories


GEO

GSE45889

ArrayExpress

E-GEOD-45889

ENA

SRP020639

BioProject

PRJNA196580

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Contact


Kyoung Jae Won

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