Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Dec 1 2012
Last update date: Jan 25 2018
Access: Public
Diseases: Neoplasms, Sarcoma, Rhabdomyosarcoma, Embryonal
Genes: MYF5, MYOG, myf5
Dataset link Expression profile of zebrafish embryonal rhabdomyosarcoma

Experimental Protocol


One-cell stage myf5-GFP/mylz2-mCherry fluorescent transgenic zebrafish were injected with rag2-kRAS12D. A subset of animals developed ERMS. Tumor cells were transplanted into syngeneic recipient animals that lacked fluorescent reporter expression. ERMS cell subfractions were isolated from transplant animals and purified cell populations obtained following two rounds of FACS. Sorted cells were 1) analyzed by microarray/RT-PCR and 2) transplanted at limiting dilution into syngeneic animals. These experiments confirm that zebrafish ERMS contain molecularly distinct cell subfractions that express high levels of myf5-GFP and exhibit difference in gene expression when compared to other ERMS cell subtypes. All four fluorescent-labeled cell populations were analyzed (n=2 tumors total).

Repositories


GEO

GSE32425

ArrayExpress

E-GEOD-32425

BioProject

PRJNA147915

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Contact


David Langenau
David M Langenau