Computational protocol: Antimycobacterial Activity of a New Peptide Polydim-I Isolated from Neotropical Social Wasp Polybia dimorpha

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Protocol publication

[…] The fractions containing Polydim-I were subjected to identification (purity and identity) through matrix-assisted laser desorption/ionization mass spectrometry time-of flight (MALDI-TOF TOF) (UltraFlex III, Bruker Daltonics, Germany) under reflector (MS) and LIFTTM (MS/MS) positive modes. Prior to analysis, the fractions were dissolved in a saturated solution of α-cyano-4-hydroxycinnamic acid matrix in acetonitrile/water/3% trifluoroacetic acid (5/4/1). Additionally, the peptides’ mass spectra and sequencing were manually interpreted using the FlexAnalysis 3.0 software (Bruker Daltonics, Germany). The monoisotopic molecular mass of the peptide was determined as the ratio between the m/z peaks in the spread profile (m/z ratio from 600 to 3,000). Moreover, similarity searches were performed using Fasta3 programs, the Expasy12 server and BLASTP13. […]

Pipeline specifications

Software tools FASTA, BLASTP
Databases ExPASy
Application Nucleotide sequence alignment
Organisms Mus musculus, Mycobacteroides abscessus, Corynebacteriales, Homo sapiens, Bacteria
Diseases Communicable Diseases, Infection