Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Nov 1 2006
Last update date: Mar 16 2012
Access: Public
Chemicals: Heme
Dataset link Microarray identification of functional modules involving Haptoglobin and/or Hemopexin

Experimental Protocol


We performed a gene expression analysis of the livers of Hp- and/or Hx-null mice compared to wild-type controls. The mice used in the following experiments, i.e. wild-type (Hp+/Hx+/), Hp-null (Hp-/-Hx+/), Hx-null (Hp+/Hx-/-), and HpHx-null (Hp-/-Hx-/-), were littermates derived by breeding F1 double heterozygous Hp+/-Hx+/- mice in the mixed genetic background C57/BLJ6 X 129Sv. We have three experimental points: Hx-null mice, Hp-null mice and HpHx-null mice. At least 5 adult mice per genotype were perfused via aorta with PBS, sacrificed and their liver pooled. Thirty microgram of total RNA were subjected to direct labeling reaction by incorporation of cyanin 3 (Cy3) or cyanin 5 (Cy5) fluorescent dyes into the cDNA by priming with oligo(dT). Four replicates were set up for each experimental point. In order to exclude artifacts resulting from different dye usage, we employed the dye-swap approach.

Repositories


GEO

GSE3656

ArrayExpress

E-GEOD-3656

BioProject

PRJNA93783

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Contact


Diego Vozzi