Dataset features

Specifications


Application: ChIP-seq analysis, BS-seq analysis, RNA-seq analysis
Number of samples: 10
Release date: May 1 2013
Last update date: Oct 24 2018
Access: Public
Taxon: Homo sapiens
Dataset link Transcriptional and Epigenetic Dynamics During Specification of Human Embryonic Stem Cells

Experimental Protocol


10 Samples in total, 5 in replicate. To better understand the interplay of epigenetic dynamics and transcription factor binding upon in vitro specification of human embryonic stem cells we profiled OCT4, SOX2 and NANOG in hESC and the endoderm master regulatory factor FOXA2 in in vitro derived endoderm cells (dEN). To gain further insights into the relation of DNA methylation and TF binding, we carried out ChIP-bisulfite sequencing for FOXA2 in dEN. Lastly, we were interested in the fate of genes bound by FOXA2 in dEN upon further differentiation and therefore differentiated dEN further towards a hepatocyte like state and performed RNA-Seq.

Repositories


GEO

GSE46130

ArrayExpress

E-GEOD-46130

ENA

SRP021134

BioProject

PRJNA197326

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