|Application:||ChIP-seq analysis, BS-seq analysis, RNA-seq analysis|
|Number of samples:||10|
|Release date:||May 1 2013|
|Last update date:||Oct 24 2018|
|Dataset link||Transcriptional and Epigenetic Dynamics During Specification of Human Embryonic Stem Cells|
10 Samples in total, 5 in replicate. To better understand the interplay of epigenetic dynamics and transcription factor binding upon in vitro specification of human embryonic stem cells we profiled OCT4, SOX2 and NANOG in hESC and the endoderm master regulatory factor FOXA2 in in vitro derived endoderm cells (dEN). To gain further insights into the relation of DNA methylation and TF binding, we carried out ChIP-bisulfite sequencing for FOXA2 in dEN. Lastly, we were interested in the fate of genes bound by FOXA2 in dEN upon further differentiation and therefore differentiated dEN further towards a hepatocyte like state and performed RNA-Seq.