Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 6
Release date: Oct 1 2014
Last update date: Jan 2 2015
Access: Public
Dataset link Large-scale identification and comparative analysis of miRNA expression profile in the respiratory tree of the sea cucumber Apostichopus japonicus during aestivation (part 1)

Experimental Protocol


In the present study, an analysis of the global profile of small RNAs was conducted using Solexa sequencing technology in non-aestivation (NA) and deep aestivation (DA) sea cucumbers. We focus on respiratory tree in the present study because it is the important site responsible for the strong metabolic rate depression seen under deep aestivating conditions and the global expression profile of mRNA from the this organ has also been constructed applying RNA-seq technology in our previous study (Zhao and Chen, unpublished data). A preliminary analysis of the functional relevance of miRNA expression in relation to hypometabolism during aestivation is presented. A miRNA microarray and RT-qPCR were both used to supplement and confirm differentially expressed miRNAs. Our findings provide important new insights into the molecular mechanisms of sea cucumber aestivation.

Repositories


GEO

GSE52224

ArrayExpress

E-GEOD-52224

BioProject

PRJNA227184

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Contact


Muyan Chen