Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Jan 10 2006
Last update date: Jul 1 2016
Access: Public
Dataset link Pac1+/+ versus Pac1-/- BMMCs_IgE-DNP 2 h

Experimental Protocol


Two genechips on independent mBMMC cultures was performed. The signal data of the .CEL files for the four Affymetrix 430A chips (2 Pac1+/+ and 2 Pac1–/–) were normalised using the RMA method 49. The ebayes function implemented in the version 1.3.12 of the Limma package (2003) of Bioconductor was used to analyse the data and P-values were adjusted for multiple testing. After conversion from logged values to the original intensities, the data was filtered to remove probe sets with mean, non-logged, intensities below 100. Transcripts with p-values less than 0.05 were considered different from Pac1+/+.

Repositories


GEO

GSE3994

ArrayExpress

E-GEOD-3994

BioProject

PRJNA104887

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Contact


Charles Mackay
Charles Reay Mackay