|Application:||Gene expression microarray analysis|
|Number of samples:||4|
|Release date:||Jan 10 2006|
|Last update date:||Jul 1 2016|
|Dataset link||Pac1+/+ versus Pac1-/- BMMCs_IgE-DNP 2 h|
Two genechips on independent mBMMC cultures was performed. The signal data of the .CEL files for the four Affymetrix 430A chips (2 Pac1+/+ and 2 Pac1–/–) were normalised using the RMA method 49. The ebayes function implemented in the version 1.3.12 of the Limma package (2003) of Bioconductor was used to analyse the data and P-values were adjusted for multiple testing. After conversion from logged values to the original intensities, the data was filtered to remove probe sets with mean, non-logged, intensities below 100. Transcripts with p-values less than 0.05 were considered different from Pac1+/+.
Charles Reay Mackay