Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 15
Release date: Nov 7 2016
Last update date: Jul 26 2018
Access: Public
Diseases: Joint Diseases, Osteoarthritis
Chemicals: Alloxan, Glucosamine
Dataset link Expression data from glucosamine-treated human synovial MH7A cells

Experimental Protocol


Human synovial MH7A cells in 6-well plates were incubated in the presence or absence of GlcN (5mM), and then stimulated with 15 pg/ml IL-1β for 13h. In some experiments, cells were preincubated with 5 mM alloxan, and then incubated with 2 mM alloxan and 5mM GlcN, followed by stimulation with IL-1β. We prepared 5 kinds of cell samples. 1; Non-treated cells. 2; IL-1β-stimulated cells. 3; GlcN-treated IL-1β-stimulated cells. 4; GlcN and alloxan-treated IL-1β-stimulated cells. 5; alloxan-treated IL-1β-stimulated cells. All expreriments were repeated three times.

Repositories


GEO

GSE72575

BioProject

PRJNA294384

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Contact


AKIMASA SOMEYA