Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 40
Release date: Jan 9 2013
Last update date: Jan 10 2013
Access: Public
Diseases: Leukemia, Myeloid, Acute
Dataset link Gene expression in signaling subsets of AML blasts induced by G-CSF

Experimental Protocol


Cryopreserved PBMC from 11 AML patinets were stimulated with G-CSF. Following stimulation cells were fixed, permeabilized and stained for selected surface markers (CD3, CD33, CD45) and and phospho-Stat3/5. After staining subsets positive and negative for phospo-Stat3/5 were sorted out for further microarray analysis. T-cells were sorted from each sample to control for the RNA quality by comparisons to RNA prepared from live T-cells sorted form healthy donors.

Repositories


GEO

GSE42140

ArrayExpress

E-GEOD-42140

BioProject

PRJNA179201

Download


Contact


Yury Goltsev