|Application:||Gene expression microarray analysis|
|Number of samples:||40|
|Release date:||Jan 9 2013|
|Last update date:||Jan 10 2013|
|Diseases:||Leukemia, Myeloid, Acute|
|Dataset link||Gene expression in signaling subsets of AML blasts induced by G-CSF|
Cryopreserved PBMC from 11 AML patinets were stimulated with G-CSF. Following stimulation cells were fixed, permeabilized and stained for selected surface markers (CD3, CD33, CD45) and and phospho-Stat3/5. After staining subsets positive and negative for phospo-Stat3/5 were sorted out for further microarray analysis. T-cells were sorted from each sample to control for the RNA quality by comparisons to RNA prepared from live T-cells sorted form healthy donors.