Computational protocol: P2Y12 receptor-mediated activation of spinal microglia and p38MAPK pathway contribute to cancer-induced bone pain

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Protocol publication

[…] All results are presented as mean ± SD. Statistical tests were performed using SPSS (Version 11.5). The changes of the PWTs and the immunohistochemistry staining of Iba-1 and p-p38MAPK in all groups were analyzed by two-way analysis of variance (ANOVA) followed by least significant difference (LSD) (when homogeneity of variance) or Tamhane’s T2 (when heterogeneity of variance) post hoc test. Unpaired T-test was used for blot analysis. The production of IL-6, IL-1β, or TNF-α cytokines was analyzed by one-way ANOVA and then compared by LSD post hoc test. Figures were prepared using Adobe Illustrator CS (Version 11.0; Adobe Systems, San Jose, CA, USA). For all experiments, P<0.05 indicates statistically significant difference. […]

Pipeline specifications

Software tools SPSS, Adobe Illustrator
Application Miscellaneous
Organisms Rattus norvegicus
Diseases Bone Neoplasms, Neoplasms
Chemicals Calcium