Computational protocol: Nuclear poly(A)-binding protein aggregates misplace a pre-mRNA outside of SC35 speckle causing its abnormal splicing

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Protocol publication

[…] Images were visualized using an Olympus BX60 microscope (Olympus Optical, Hamburg, Germany), digitalised using a CCD camera (Photometrics CoolSNAP fx; Roper Scientific) and analyzed using MetaView image analysis system (Universal Imaging, Downington, PA, USA), MetaMorph imaging system (Roper Scientific, Tucson, AZ, USA) software, and ImageJ 1.44o (http://imagej.nih.gov/ij) for quantification analysis. […]

Pipeline specifications

Software tools MetaMorph, ImageJ
Application Microscopic phenotype analysis
Organisms Homo sapiens
Chemicals Calcium