Computational protocol: BhbZIP60 from Resurrection Plant Boea hygrometrica Is an mRNA Splicing-Activated Endoplasmic Reticulum Stress Regulator Involved in Drought Tolerance

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Protocol publication

[…] The RNA structure was predicted using M-fold program with default settings (). The amino acid sequences were obtained from NCBI (National Center for Biotechnology Information) database and aligned using the ClustalX 2.0 program with default settings and adjusted manually using GeneDoc software. The subcellular localization was predicted with the online software . The phylogenetic tree was constructed using the Neighbor Joining (NJ) method in Clustalx1.83 and viewed using the software MEGA6 with the following parameters: bootstrap (1,000 replicates; random seed), pair-wise deletion, and Poisson correction, according to the method of . […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Organisms Dorcoceras hygrometricum, Saccharomyces cerevisiae
Chemicals Abscisic Acid, Inositol, Mannitol, Tunicamycin