Computational protocol: Fibulin-1 is epigenetically down-regulated and related with bladder cancer recurrence

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Protocol publication

[…] Genomic DNA was isolated using QIAamp DNA Mini Kit (Qiagen) and bisulfite modification of the genomic DNA was carried out using an Epitect Bisulfite Kit (Qiagen) according to the manufacturer’s instructions. Methylation Specific PCR (MSP) primers were designed with Methprimer (http://epidesigner.com). Pyrosequencing for five CpG sites in the promoter region was performed on a PyroMark Q96 instrument (Qiagen) according to manufacturer’s protocol, as described previously []. Record was analyzed by the manufacturer’s software. All the primer sequences were listed in Additional file : Table S1. […]

Pipeline specifications

Software tools methPrimer, EpiDesigner
Application BS-seq analysis
Organisms Mus musculus, Homo sapiens
Diseases Urinary Bladder Neoplasms, Neoplasms