|Number of samples:||13|
|Release date:||Aug 13 2013|
|Last update date:||Jul 20 2018|
|Dataset link||Cohesin-based chromatin interactions enable regulated gene expression within pre-existing architectural compartments.|
The chromatin binding of cohesin, the cohesin-loading loading complex and Mediator was analysed by ChIP-sequencing in thymocytes for the respective subunits Rad21, NIPBL and Med1. The role of the cohesin complex in transcriptional regulation was investigated using RNA-sequencing in wild type and Rad21 KO thymocytes, while the global role of cohesin in chromatin organisation was investigated by Hi-C in wild type and Rad21 KO thymocytes. This approach allowed us to integrate the binding of cohesin and its associated factors with changes in transcription and chromatin organisation upon cohesin depletion.