Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 13
Release date: Aug 13 2013
Last update date: Jul 20 2018
Access: Public
Dataset link Cohesin-based chromatin interactions enable regulated gene expression within pre-existing architectural compartments.

Experimental Protocol


The chromatin binding of cohesin, the cohesin-loading loading complex and Mediator was analysed by ChIP-sequencing in thymocytes for the respective subunits Rad21, NIPBL and Med1. The role of the cohesin complex in transcriptional regulation was investigated using RNA-sequencing in wild type and Rad21 KO thymocytes, while the global role of cohesin in chromatin organisation was investigated by Hi-C in wild type and Rad21 KO thymocytes. This approach allowed us to integrate the binding of cohesin and its associated factors with changes in transcription and chromatin organisation upon cohesin depletion.

Repositories


GEO

GSE48763

ENA

SRP026700

BioProject

PRJNA211477

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Contact


Andre Faure