Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Aug 16 2016
Last update date: Aug 23 2018
Access: Public
Diseases: Gastrointestinal Diseases, Enterocolitis, Necrotizing
Genes: YY1
Dataset link A YY1-dependent increase in aerobic metabolism is indispensable for intestinal organogenesis

Experimental Protocol


Mouse embryos of the same genotypes were pooled (up to 2) to obtain 2 biological replicates of YY1f/f and Yy1f/f; Shh-Cre. Epcam positive epithelium cells were enriched using magnetic selection from Small intestines of E15.5 embryos(proximal stomach to distal caecum). Cells were then dissolved in Trizol and RNA prepared by ethanol precipitation using 10ug LPA. RNA was then processed for microarray on Affymetrix Mouse 430_2 arrays according to manufacturer’s instructions.

Repositories


GEO

GSE85602

BioProject

PRJNA338925

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Contact


Michael Verzi