|Number of samples:||1|
|Release date:||Mar 1 2013|
|Last update date:||Nov 27 2018|
|Dataset link||GRO-seq from HCT116 cells|
Nuclei were prepared from HCT116 cells (treated for 1hr with DMSO as control for additional GRO-seq experiments to be reported separately). Transcription run-on was performed (as per Core, L.J., Waterfall, J.J., and Lis, J.T. (2008). Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters. Science 322, 1845-1848) and nascent RNAs were purified and sequenced.