Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 1
Release date: Mar 1 2013
Last update date: Nov 27 2018
Access: Public
Diseases: Colonic Neoplasms
Dataset link GRO-seq from HCT116 cells

Experimental Protocol


Nuclei were prepared from HCT116 cells (treated for 1hr with DMSO as control for additional GRO-seq experiments to be reported separately). Transcription run-on was performed (as per Core, L.J., Waterfall, J.J., and Lis, J.T. (2008). Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters. Science 322, 1845-1848) and nascent RNAs were purified and sequenced.

Repositories


GEO

GSE38140

ArrayExpress

E-GEOD-38140

ENA

SRP013319

BioProject

PRJNA167277

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