Computational protocol: Identification and functional characterisation of the promoter of the calcium sensor gene CBL1 from the xerophyte Ammopiptanthus mongolicus

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Protocol publication

[…] DNA sequences were analysed by software DNAMAN, and the promoter elements were analysed by PLACE http://www.dna.affrc.go.jp/PLACE/ and PLANTCARE http://bioinformatics.psb.ugent.be/webtools/plantcare/html/. Sequence alignments were created with the program DIALIGN. Differences in GUS activity among treatment groups were tested with analyse of variance (ANOVA) in SPSS 16.0 […]

Pipeline specifications

Software tools DNAMAN, DIAlign
Databases PlantCARE
Application MS-based untargeted metabolomics
Organisms Nicotiana tabacum, Arabidopsis thaliana
Chemicals Calcium