Computational protocol: Upregulation of UCP2 by Adiponectin: The Involvement of Mitochondrial Superoxide and hnRNP K

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Protocol publication

[…] All results were derived from at least three sets of experiments. The density of protein bands in Western blotting results were quantitatively analyzed by ImageJ software for calculating the expression ratios against loading control. Representative Western blotting images were shown. Values are expressed as mean ± SEM. The statistical calculations were performed with the Statistical Package for the Social Sciences version 11.5 software package (SPSS, Inc., Chicago, IL). Differences between groups were determined by Student's t-test. Comparisons with P<0.05 were considered as statistically significant. […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Mus musculus
Diseases Liver Diseases, Chemical and Drug Induced Liver Injury
Chemicals Cycloheximide, Dactinomycin, Rotenone, Superoxides