Dataset features


Application: Gene expression microarray analysis
Number of samples: 20
Release date: Jan 13 2013
Last update date: Apr 15 2013
Access: Public
Diseases: Insulinoma, Neoplasms, Tumor Lysis Syndrome
Dataset link Gene expression profiling of primary canine insulinomas and their metastases

Experimental Protocol

From 1993 to 2009, ten histologically confirmed primary insulinomas (INS) and their accompanying metastases were obtained from dogs that were presented at the Department of Clinical Sciences of Companion Animals of the Faculty of Veterinary Medicine of Utrecht University. Tumors were resected by experienced surgeons, and fragments (5-15 mm diameter) for RNA extraction were carefully removed from the central portion of the tumors to avoid contamination with normal adjacent tissue. Tissue specimens collected for RNA isolation were snap-frozen in liquid nitrogen immediately after surgical removal and stored at 70oC. Various clinical and pathological parameters were evaluated retrospectively for the dogs included in this study. Half of both the primary insulinoma samples, as well as half of the metastasis samples was labelled with Cy3, while the other half of both groups was labelled with Cy5. A common reference cRNA pool consisting of equal quantity of cRNA from all 20 tumor samples was used to hybridize against each sample on the array.










Floryne Buishand