|Application:||DNA methylation array analysis|
|Number of samples:||190|
|Release date:||Oct 18 2010|
|Last update date:||Jan 2 2015|
|Diseases:||Leukemia, Plasma Cell, Multiple Myeloma, Precursor Cell Lymphoblastic Leukemia-Lymphoma|
|Dataset link||Aberrant global methylation patterns affect the molecular pathogenesis and prognosis of human multiple myeloma|
Bone marrow aspirates were obtained after informed consent. B cells (n=6) from normal individuals were selected from peripheral blood using CD19. Plasma cells (PC) from non-myeloma patients (normal plasma cell controls, n=3) as well as MGUS (n=4) and presentation myeloma patients (n=161) were selected to a purity of >90% using CD138 microbeads and magnet-assisted cell sorting (Miltenyi Biotech, Bisley, UK). Samples from PCL patients (n=7) were not CD138 selected but contained >90% plasma cell infiltration as determined by microscopy. In order to achieve a sufficient quantity of DNA some normal plasma cell control samples were pooled.