Dataset features

Specifications


Application: DNA methylation array analysis
Number of samples: 190
Release date: Oct 18 2010
Last update date: Jan 2 2015
Access: Public
Diseases: Leukemia, Plasma Cell, Multiple Myeloma, Precursor Cell Lymphoblastic Leukemia-Lymphoma
Dataset link Aberrant global methylation patterns affect the molecular pathogenesis and prognosis of human multiple myeloma

Experimental Protocol


Bone marrow aspirates were obtained after informed consent. B cells (n=6) from normal individuals were selected from peripheral blood using CD19. Plasma cells (PC) from non-myeloma patients (normal plasma cell controls, n=3) as well as MGUS (n=4) and presentation myeloma patients (n=161) were selected to a purity of >90% using CD138 microbeads and magnet-assisted cell sorting (Miltenyi Biotech, Bisley, UK). Samples from PCL patients (n=7) were not CD138 selected but contained >90% plasma cell infiltration as determined by microscopy. In order to achieve a sufficient quantity of DNA some normal plasma cell control samples were pooled.

Repositories


GEO

GSE21304

ArrayExpress

E-GEOD-21304

BioProject

PRJNA126573

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Contact


Brian Walker