Computational protocol: SCARN a Novel Class of SCAR Protein That Is Required for Root-Hair Infection during Legume Nodulation

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Protocol publication

[…] The A. thaliana protein sequences were obtained from TAIR ( and the L. japonicas protein sequence were obtained from website ( Version 3.0, and other legume SCAR protein sequences were obtained from Phytozome 10.1 ( All the protein sequences are imported into MEGA6.0 [] for complete alignment using ClustalW2 ( The phylogenetic tree was built using MEGA6.0 [] and using the neighbor-joining method with the bootstrapping value set at 1000 replications. […]

Pipeline specifications

Software tools MEGA, Clustal W
Databases Phytozome
Application Phylogenetics
Organisms Lotus japonicus, Arabidopsis thaliana, Mesorhizobium loti
Diseases Thyroid Nodule