Computational protocol: SCARN a Novel Class of SCAR Protein That Is Required for Root-Hair Infection during Legume Nodulation

Similar protocols

Protocol publication

[…] The A. thaliana protein sequences were obtained from TAIR (http://www.arabidopsis.org/) and the L. japonicas protein sequence were obtained from miyakogusa.jp website (http://www.kazusa.or.jp/lotus/) Version 3.0, and other legume SCAR protein sequences were obtained from Phytozome 10.1 (http://phytozome.jgi.doe.gov/pz/portal.html). All the protein sequences are imported into MEGA6.0 [] for complete alignment using ClustalW2 (http://www.ebi.ac.uk/Tools/msa/clustalw2/). The phylogenetic tree was built using MEGA6.0 [] and using the neighbor-joining method with the bootstrapping value set at 1000 replications. […]

Pipeline specifications

Software tools MEGA, Clustal W
Databases Phytozome
Application Phylogenetics
Organisms Lotus japonicus, Arabidopsis thaliana, Mesorhizobium loti
Diseases Thyroid Nodule