|Application:||Gene expression microarray analysis|
|Number of samples:||27|
|Release date:||Jan 1 2013|
|Last update date:||Oct 29 2018|
|Diseases:||Asthma, Bacterial Infections, Crohn Disease, Dermatitis, Atopic|
|Genes:||NOD2, TNFAIP3, IER3|
|Dataset link||NOD2 and desease associated variant NOD2-L1007fsinsC dependent genomewide transcriptional regulation in stable Flp-In HEK cells|
To elucidate the MDP-induced activation program we generated isogenic HEK293 cells stably expressing wildtype NOD2 or NOD2L1007fsinsC using a FRT-recombinase based approach. Cells carrying the inserted vector cassette were used as controls (mock-transfectant). To comprehensively analyze NOD2-mediated innate immune responses we analyzed transcriptomal signature patterns using genome-wide cDNA microarrays. Samples were harvested from cell cultures under normal growth conditions 0 h, 2 h and 6 h after MDP‑ stimulation of the cells.