Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 27
Release date: Jan 1 2013
Last update date: Oct 29 2018
Access: Public
Diseases: Asthma, Bacterial Infections, Crohn Disease, Dermatitis, Atopic
Chemicals: Acetylmuramyl-Alanyl-Isoglutamine
Genes: NOD2, TNFAIP3, IER3
Dataset link NOD2 and desease associated variant NOD2-L1007fsinsC dependent genomewide transcriptional regulation in stable Flp-In HEK cells

Experimental Protocol


To elucidate the MDP-induced activation program we generated isogenic HEK293 cells stably expressing wildtype NOD2 or NOD2L1007fsinsC using a FRT-recombinase based approach. Cells carrying the inserted vector cassette were used as controls (mock-transfectant). To comprehensively analyze NOD2-mediated innate immune responses we analyzed transcriptomal signature patterns using genome-wide cDNA microarrays. Samples were harvested from cell cultures under normal growth conditions 0 h, 2 h and 6 h after MDP‑ stimulation of the cells.

Repositories


GEO

GSE22611

ArrayExpress

E-GEOD-22611

BioProject

PRJNA128351

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