Computational protocol: Natural Variation in an ABC Transporter Gene Associated with Seed Size Evolution in Tomato Species

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Protocol publication

[…] A 38 kb segment, delimited by markers dABC20665 and S106indel1 and known from progeny analyses to encompass the Sw4.1 QTL, was also sequenced via PCR from the genome of S. pimpinellifolium LA1589 – the small-seeded parent of the original mapping population. The objective of the sequencing was to identify polymorphisms that might be causal to the Sw4.1 QTL. Sequence analysis was performed with DNASTAR Lasergene software and alignments with BioEdit version using ClustalW. […]

Pipeline specifications

Software tools DNASTAR Lasergene, BioEdit, Clustal W
Application Sanger sequencing
Organisms Solanum lycopersicum, Arabidopsis thaliana, Homo sapiens