Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 21
Release date: Jul 1 2018
Last update date: Nov 27 2018
Access: Public
Diseases: Dental Caries
Dataset link The Nonstop decay and the RNA silencing systems operate cooperatively in plants

Experimental Protocol


To verify if NSD is also involved in the degradation of 5’ cleavage products of endogenous miRNA targets, RNA-seq libraries (in triplicates) were prepared with random primers from ribo-depleted RNA samples, which were isolated from Pelota- and Control silenced N. benthamiana leaves (RNA-seq1 and RNA-seq2 experiments) and from wild type (Columbia) and pelota1 and hbs1 T-DNA mutant Arabidopsis thaliana leaves (Arabidopsis RNA-seq). N. benthamiana sequencing was repeated twice.

Repositories


GEO

GSE99805

ENA

SRP108830

BioProject

PRJNA389675

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Contact


Daniel Silhavy