Computational protocol: A novel blood feeding detoxification pathway in Nippostrongylus brasiliensis L3 reveals a potential checkpoint for arresting hookworm development

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Protocol publication

[…] BlastP (v2.2.28, was used to identify Nb protein sequences obtained from the L3 & “L5” adult secretomes [] presenting high similarity to groups of selected target proteins of interest. BlastX (v2.2.28, was used to do the same for proteins translated from transcripts assembled from the iL3 transcriptome []. Top-scoring hits with alignments covering at least 95% of the Nb proteins were considered for further analysis. Two groups of target proteins of interest were defined: one including homologs of the Na-APR1 protein (see list below), and one including homologs of Na-GST1 protein (see list below). With these targets, 1 and 11 putative Nb protein homologs were manually identified from the Blast results, respectively. Proteins from each group were then aligned using Clustal Omega (v1.2.3, with default settings for protein alignment. Multiple sequence alignments were visualised using the JalView 2 Desktop application ( For the GST1 group, amino acids were visually coloured based on the ClustalX scheme. Molecular phylogenies were generated using Clustal Omega’s Phylogeny program using UPGMA clustering with distance correction (, and were used to order sequences in the alignments. Finally, sequence features of interest were manually highlighted in the alignments. […]

Pipeline specifications

Software tools BLASTP, BLASTX, Clustal Omega, Jalview, Clustal W
Application Phylogenetics
Organisms Homo sapiens, Necator americanus, Canis lupus familiaris, Mus musculus, Caenorhabditis elegans, Rattus norvegicus
Diseases Anemia, Hookworm Infections