Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 23
Release date: May 31 2009
Last update date: Oct 29 2018
Access: Public
Diseases: Carcinoma, Lymphoma, Non-Hodgkin, Neoplasms, Ovarian Diseases
Dataset link PAX2: A Potential Biomarker for Low Malignant Potential Ovarian Tumors and Low-Grade Serous Ovarian Carcinomas

Experimental Protocol


We used RNA from 3 normal human ovarian surface epithelia (HOSE) and from 10 low-grade and 10 high-grade serous ovarian carcinoma samples to perform gene expression profiling. Using real-time reverse-transcription polymerase chain reaction (RT-PCR), we evaluated changes in PAX2 mRNA expression in cDNA created from RNA extracted from an independent set of ovarian tissue samples (7 LMP tumors and 17 low-grade and 23 high-grade serous carcinomas). We also examined PAX2 expression using Western blot analysis of protein extracted from a set of ovarian LMP and low- and high-grade carcinoma tissue samples. Additionally, we used immunohistochemistry (IHC) to validate PAX2 overexpression in a third independent set of paraffin ovarian tissue sections from 17 LMP tumors and 16 low- and 257 high-grade carcinomas.

Repositories


GEO

GSE14001

ArrayExpress

E-GEOD-14001

BioProject

PRJNA112503

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Contact


Kwong-Kwok Wong

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