Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 15
Release date: Mar 25 2015
Last update date: Jul 1 2015
Access: Public
Diseases: Neoplasms, Prostatic Neoplasms
Genes: PROM1, CBX8
Dataset link NanoString nCounter GX Cancer Reference Kit and the custom made ITESM (14 genes associated with prostate cancer)

Experimental Protocol


PC3 prostasphere cultures were sampled on day 0 (Parental monolayers) (EPI_1), day 4 (EPI_2), day 8 (EPI_3), and day 12 (EPI_4) of culture. Day 12 prostaspheres were also enzymatically dissociated and sorted via MACS using the Anti-CD133/2 (293C3)-PE Antibody (Miltenyi) into CD133+ (EPI_P) and CD133- (EPI_N) fractions. Total RNA was extracted from these fractions and analyzed using the nCounter GX Cancer Reference kit and the custom made ITESM CodeSet from NanoString Technologies. Three independent experiments were carried out in parallel.

Repositories


GEO

GSE67248

BioProject

PRJNA279320

Download


Contact


Roberto Portillo Lara

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