Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 51
Release date: Feb 5 2018
Last update date: Nov 7 2018
Access: Public
Dataset link Two distinct transcription termination modes dictated by promoters

Experimental Protocol


RNAi-treated, polyA-selected RNA-seq data: 3 replicates of mock and xrn-2 RNAi were analyzed, 2 of which can be found under series GSE79994 (1 included here). ChIP-seq: 2 replicates of ChIP and input samples for each factor/condition were analyzed, including untagged (control) strains for XRN2 ChIP. RNAi-treated, ribosomal RNA-depleted RNA-seq: 2 replicates of xrn-2 RNAi and one replicate of mock were analyzed. TS-mutant RNA-seq: one replicate of WT and xrn-2(xe31) RNA per timepoint (hours after temperature shift) were analyzed. Timepoints 06h and 10h were re-sequenced at approximately 4-fold deeper coverage. Catalytic-dead mutant RNA-seq: one replicate of WT and xrn-2cd;xrn-2(xe31) RNA per timepoint (06h and 10h after temperature shift) were analyzed.

Repositories


GEO

GSE97775

ENA

SRP103917

BioProject

PRJNA382843

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Contact


Sarah Carl