Computational protocol: Allele frequencies of 15 STR loci in Bosnian and Herzegovinian population

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[…] Allele frequencies, matching probability (MP), power of discrimination (PD), power of exclusion (PE), and typical paternity index (TPI) were calculated within Microsoft Excel workbook template – PowerStats (). Powermarker version 3.25 was used for estimation of allele number (AN) (), deviation from Hardy–Weinberg equilibrium (), polymorphism information content (), and observed and expected heterozygosity (). Exact test of population differentiation () was calculated within Arlequin version 3.5.1.2 (). After Bonferroni’s correction, statistically significant deviation from Hardy–Weinberg equilibrium was considered as P < 0.01 and P < 0.001 for the population differentiation test. The number of effective alleles (AE) was estimated as 1/ ∑ pi2, where p denotes the allele frequency for a particular locus. Ratio of effective and detected numbers of alleles and its statistical significance was calculated as suggested by Pojskic et Kalamujic () with Alleles Ratio, a Microsoft Excel workbook template (). A Z-score of P < 0.01 was considered statistically significant. In order to estimate genetic distance among populations, we have implemented Dsw method proposed by Shriver et al (). The neighbor-joining dendrogram was constructed based on genetic distance results (). These calculations were performed using POPTREE software (). […]

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