|Application:||Gene expression microarray analysis, miRNA array analysis|
|Number of samples:||9|
|Release date:||Oct 27 2009|
|Last update date:||Jun 26 2012|
|Dataset link||Sensitivity and linear dynamic range of miRNA detection|
The linear dynamic range and sensitivity of the microarray was measured by hybridizing dilution series of a Universal Reference (UR), an equimolar mixture of synthetic miRNAs. The UR was hybridized with 10,000 to 1 amol of each individual miRNA. Each individual miRNA and 1 fmol of each of 18 RNA oligonucleotides reverse complement to miRControl 3 probes was fluorescently labelled by 3’ ligation. The RNA mix was hybridized in a dual colour approach to microarrays versus a second labelled synthetic miRNA pool.