Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 9
Release date: Oct 27 2009
Last update date: Jun 26 2012
Access: Public
Genes: CD34
Dataset link Sensitivity and linear dynamic range of miRNA detection

Experimental Protocol


The linear dynamic range and sensitivity of the microarray was measured by hybridizing dilution series of a Universal Reference (UR), an equimolar mixture of synthetic miRNAs. The UR was hybridized with 10,000 to 1 amol of each individual miRNA. Each individual miRNA and 1 fmol of each of 18 RNA oligonucleotides reverse complement to miRControl 3 probes was fluorescently labelled by 3’ ligation. The RNA mix was hybridized in a dual colour approach to microarrays versus a second labelled synthetic miRNA pool.

Repositories


GEO

GSE15834

ArrayExpress

E-GEOD-15834

BioProject

PRJNA116763

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Contact


Ute Bissels