Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: May 23 2017
Last update date: Aug 22 2018
Access: Public
Diseases: Carcinoma, Hepatocellular, Liver Diseases
Chemicals: Oleic Acid
Dataset link Role of caveolin-1 in hepatocellular carcinoma arising from non-alcoholic fatty liver disease

Experimental Protocol


The aim of this study is to identify an important factor in HCC developing from NAFLD (NAFLD-HCC) and to elucidate its function. Human HCC tissues and the adjacent non-HCC tissues were separately excised from the resected specimens from 3 NAFLD-HCC patients and 3 patients with HCC arising from hepatitis C (HCV-HCC). Samples were snap-frozen with RNA later (Applied Biosystems, Grand Island, NY) in liquid nitrogen and stored at -80℃. Total RNA was extracted from the frozen resected sample using an RNeasy Mini Kit (Quiagen, Valencia, CA, USA). The intensity and quantity of total RNA was assessed with a Nanodrop ND-1000 spectrometer (Nanodrop Technologies, Wilmington, DE, USA). The gene expression profile of HCV-HCC and NAFLD-HCC was determined by Affymetrix Gene Chip Human Gene 2.0 ST array.

Repositories


GEO

GSE99131

BioProject

PRJNA387398

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Contact


Takanori Sakaguchi