Computational protocol: Polyglutamine length-dependent toxicity from α1ACT in Drosophila models of spinocerebellar ataxia type 6

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Protocol publication

[…] All fluorescence images were taken with an Olympus BX53 microscope and CellSens software (Olympus, Waltham, MA, USA). The same objective (10×) and camera settings (ISO 200, 500 ms capture time for each sample) were used for all images. Image capture and quantification (ImageJ, NIH) were conducted by separate investigators. Control and experimental flies were all imaged on the same day. ImageJ's freehand tool was used to outline and to quantify fluorescence readings from each fly eye. […]

Pipeline specifications

Software tools cellSens, ImageJ
Application Microscopic phenotype analysis
Organisms Drosophila melanogaster, Homo sapiens, Mus musculus
Diseases Retinal Degeneration, Neurodegenerative Diseases, Spinocerebellar Ataxias, Drug-Related Side Effects and Adverse Reactions
Chemicals Calcium