Computational protocol: Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators

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Protocol publication

[…] Sequencing data was processed using FLASH2 ( to overlap forward and reverse reads and merge paired reads into a single long read. For amplicons containing a 5-nt barcode, we demultiplexed merged reads with the FASTX barcode splitter by identifying barcodes at the beginning or the reverse complement barcodes at the end of sequence reads, allowing for one mismatch. Processed fastq files were analyzed with the CRISPResso online tool ( using default settings. […]

Pipeline specifications