Dataset features

Specifications


Dataset type: Non-coding RNA profiling by high throughput sequencing
Number of samples: 18
Release date: Dec 31 2018
Last update date: Feb 16 2019
Access: Public
Dataset link Antiviral and anti-inflammatory properties of novel anti-HIV candidate ABX464 promotes specifics RNA splicing while preserving cellular RNA integrity.

Experimental Protocol


We used buffy coats from HIV-negative individuals were obtained from the local blood donation center, then human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll (Histopaque, Sigma) gradient centrifugation. Cells were infected with 80 ng of p24/106 cells of the YU-2 strain for 4 to 6 hours and then rinsed with PBS before medium renewal.

Repositories


GEO

GSE120109

ENA

SRP162020

BioProject

PRJNA491672

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Contact


Laurent MANCHON