Computational protocol: Autophagy promotes degradation of internalized collagen and regulates distribution of focal adhesions to suppress cell adhesion

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[…] We used endogenous paxillin as an FA marker (; ). Image analysis was performed using ImageJ software (Wayne Rasband; the Research Services Branch, National Institute of Mental Health, Bethesda, MD, USA) after appropriate thresholding, as previously described (). [...] All statistical analyses were performed using JMP Pro 11 (SAS Institute Inc., NC, USA). The statistical significance of differences between groups was evaluated by an unpaired two-tailed Student's t-test with Welch's correction, a Mann–Whitney U test, F test repeated-measures analysis of variance (ANOVA), or ANOVA with Tukey's post hoc test. P<0.05 was considered statistically significant in all experiments. […]

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